Journal: bioRxiv

Article Title: ZNF768 regulates expression of E2F1 protein to drive G0/G1 transition and cell cycle progression

doi: 10.64898/2026.08.03.742597

Figure Lengend Snippet: A Western blots were performed on mitotic lysates generated from the panel of cell lines depleted of ZNF768 with inducible sh_ZNF768_1 ( n =3). B Representative immunofluorescence image of mitotic inducible HeLa cells after 48 hours doxycycline induction. Cells were stained for PLK1 (green), CENPC (grey) and with Hoechst (blue). C Quantification of the average relative intensity of PLK1 at kinetochores from ( B ) using previous inducible cancer cell lines ( n =3). D Representative stills of time-lapse movies of inducible HeLa cells after a 48 hours ZNF768 knockdown. For imaging, cells were synchronized in mitosis after release from a double thymidine block and incubated with SiR-DNA to visualize the chromatin. E Quantification of the average nuclear envelope breakdown to the anaphase onset duration in the indicated cell lines ( n = n=3 for all cell lines except for the HeLa, n=4). F Representative live cell images of mitotic defects observed after 48 hour ZNF768 depletion in HeLa cells. G Quantification of mitotic defects listed in F in MDA-MB-231, HeLa, HCT-116 and U87 cell lines after 48 hour ZNF768 depletion ( n =3). In all panels, data represent the mean ± SEM. In panel C , E and G , significance was determined by unpaired t test.

Article Snippet: For live-cell imaging, cells were washed with PBS and treated with SiR-DNA dye (CY-SC007; Cytoskeleton) at 300 nM for 1 h prior to imaging.

Techniques: Western Blot, Generated, Immunofluorescence, Staining, Knockdown, Imaging, Blocking Assay, Incubation

Journal: The Journal of Clinical Investigation

Article Title: High PRMT5 levels, maintained by KEAP1 inhibition, drive chemoresistance in high-grade serous ovarian cancer

doi: 10.1172/JCI184283

Figure Lengend Snippet: ( A ) Schematic displays TMAs used for PRMT5 staining. Figure was created with BioRender.com. ( B ) Representative IHC images show QuPath annotation of tumor stroma and epithelial cells (left), and a violin plot shows PRMT5 staining intensity in stroma versus tumor epithelial cells (right). P value was determined by Wilcoxon rank-sum test. Original magnification: ×5. ( C ) Representative IHC images show PRMT5 staining in primary and recurrent tumors. Original magnification: ×5. ( D ) The density plot displays H-scores for PRMT5 staining in primary tumors (left), and the dot plot shows PRMT5 levels (H-score) between primary and recurrent tumors for the primary tumors with low PRMT5 staining (right). P values were quantified by 2-tailed, paired Student’s t test. ( E ) Western blots show PRMT5 and SDMA levels in chemo-naive and -resistant isogenic cell line pairs. Noncontiguous lanes on different blots have been separated by thin dashed lines. ( F ) Western blots show PRMT5 expression after different doses of Dox induction (72 hours) in chemo-naive OVCAR4 cells. Carboplatin-resistant OVCAR4 cells were used to determine Dox levels for overexpression. ( G ) Line plots show the relative apoptosis rate of OVCAR4 cells treated with the indicated doses of Dox. Cells were subjected to 40 μM carboplatin, and apoptosis (caspase 3/7 activity) was monitored by the IncuCyte live-cell imaging platform for 72 hours. Data are shown as the mean ± SEM ( n = 3). P values were quantified by 1-way ANOVA with Dunnett’s multiple-comparison test. ( H ) Western blots show PRMT5 levels in OVCAR4 cells expressing sgRNAs targeting PRMT5 promoter. ( I ) Line plots show the relative apoptosis rates of OVCAR4 cells expressing the indicated sgRNAs. Cells were treated with 20 μM carboplatin, and apoptosis was monitored over 72 hours using the IncuCyte live-cell imaging platform. Data are shown as the mean ± SEM ( n = 3). P values were determined by 1-way ANOVA with Dunnett’s multiple-comparison test.

Article Snippet: The following day, treatments were administered using a drug of interest mixed with a 1:1,000 dilution of caspase 3/7 dye (10403, Biotum) and a 1:2,000 dilution of SiRDNA nuclei stain (CY-SC007, Spirochrome).

Techniques: Staining, Western Blot, Expressing, Over Expression, Activity Assay, Live Cell Imaging, Comparison